SARS-CoV-2 NGS for Surveillance Training 2022


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The NGS Academy forms part of the Africa CDC Pathogen Genomics Initiative (PGI). This initiative is funded by the Bill & Melinda Gates Foundation. Our training initiatives are carried out in partnership with the African Society for Laboratory Medicine (ASLM).

Course overview:

The course is designed for individuals looking to broaden their knowledge and build skills in the use of next generation sequencing (NGS) for SARS-CoV-2 surveillance particularly those who work in the laboratory on sample preparation, sequencing and basic data analysis.

Intended audience:

Personnel of national public health institutions on the African continent, carrying out the sequencing and bioinformatics analysis of SARS-CoV-2.

The course will cover:

Logistics:


  1. Venue of course: Online through Zoom
  2. Dates for the course: Self paced
  3. Participation: The course materials are openly available for self paced learning. Interactive online sessions with the course instructors will be organized as required for specific participants that are due to attend in person wet-lab training.
Objectives and expected outcomes:
  1. The training will focus on key topics such as sample preparation, sequencing, data analysis, curation and sharing, basic phylogenetics and epidemiology in a set of 7 independent modules. The topics are all related to the application of genomics to epidemiological investigations and public health response to SARS-CoV-2. The modules are designed to ensure an engaging online learning experience.
Course curriculum:
  1. Pre-course workshop
  2. Module 1 Introduction to Genomic Epidemiology and NGS
  3. Module 2 Wetlab & Sample preparation
  4. Module 3 ONT workflow
  5. Module 4 Illumina workflow
  6. Module 5 Phylogenetics
  7. Module 6 Data Curation and sharing
  8. Module 7 Presenting Data to Inform Policy

Course schedule and Materials:

Date Course materials Total Time (HH:MM:SS) Training partner
2022
Session 1
  • 03:00:00
  • H3ABioNet/The Carpentries

Date Course materials Total Time (HH:MM:SS) Training partner
2022
Module 1 Session 1      
  • 00:56:42
  • US CDC
  • MRIGlobal
  • Illumina

Date Course materials Total Time (HH:MM:SS) Training partner
2022
Module 2 Session 1      
  • 00:37:29

  • MRIGlobal
  • Illumina
2022
Module 2 Session 2
  • 00:10:00
  • MRIGlobal
  • Illumina

Date Course materials Total Time (HH:MM:SS) Training partner
2022
Module 4 Session 1      
  • 00:37:20
  • MRIGlobal
  • Illumina
  • CZ Biohub
2022
Module 4 Session 2
  • 00:15:00
  • MRIGlobal
  • Illumina
  • CZ Biohub
2022
Module 4 Session 3
  • 00:15:00
  • MRIGlobal
  • Illumina
  • CZ Biohub

Date Course materials Total Time (HH:MM:SS) Training partner
2022
Module 6 Session 1      
  • 00:15:00
  • KEMRI-Wellcome Trust
  • PHA4GE/SANBI
2022
Module 6 Session 2
  • 00:15:00
  • KEMRI-Wellcome Trust
  • PHA4GE/SANBI
2022
Module 6 Session 3
  • 00:15:00
  • KEMRI-Wellcome Trust
  • PHA4GE/SANBI